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Vibrio tapetis, the Causative Agent of Brown Ring Disease, Forms Biofilms with Spherical Components ArchiMer
Rodrigues, Sophie; Paillard, Christine; Le Pennec, Gael; Dufour, Alain; Bazire, Alexis.
Vibrio tapetis is a marine bacterium causing Brown Ring Disease (BRD) in the Manila clam Ruditapes philippinarum. V. tapetis biofilm formation remains unexplored depite the fact that it might be linked to pathogenicity. Our objectives were to characterize the in vitro biofilm formation of V tapetis and evaluate the effects of culture conditions. Biofilm structure and its matrix composition were examined by confocal laser scanning microscopy and scanning electron microscopy. V tapetis was able to form biofilms on a glass substratum within 24 h. Polysaccharides and extracellular DNA of the biofilm matrixes were differently distributed depending on the V tapetis strains. Spherical components of about 1-2 mu m diameter were found at the biofilm surface. They...
Tipo: Text Palavras-chave: Vibrio tapetis; Biofilm; Brown Ring Disease; Ruditapes philippinarum; Spherical components; Temperature effect; Salinity effect.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00307/41783/42796.pdf
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